BAS Biotechnology (Shenzhen) Co., Ltd. was established in 2012, focusing on the research, production, and sales of coenzyme products utilizing whole enzyme catalysis technology. The company has developed five major technical platforms for research and development, including protein expression, enzyme modification, enzyme purification and immobilization, enzyme catalytic reaction condition design and optimization, and product purification.

Protein Expression Platform

The protein expression platform utilizes three host organisms: Escherichia coli, yeast, and Streptomyces. The development process for protein expression in Escherichia coli involves the following steps:

1. Selection of promoters, tags, and vectors.

2. Screening for expression efficiency.

3. Optimization of expression temperature and inducer dosage in shake flasks.

4. Optimization of fermentation process in 1-20L bioreactors.

Enzyme Modification Platform

The core research team possesses extensive experience in biocatalysis, structural biology, and directed evolution, with publications in renowned journals such as PNAS, PLoS Pathogens, FEBS J, JMB, JAFC, and Biotechnology for Biofuels. Enzyme engineering techniques, including (semi) rational design, directed evolution, and multi-enzyme fusion, are employed to alter enzyme expression levels, thermal stability, substrate specificity, and catalytic efficiency. These techniques have been applied in various projects involving coenzyme factors, stevia glycosides RD, and allose.

Enzyme Purification and Immobilization Platform

For in vitro enzyme catalysis, BAS has independently developed various purification resins and immobilization resins suitable for industrial production. Common functional groups include NTA, IDA, epoxy, amino, and composite groups. Different resin materials can be selected based on protein properties and reaction conditions. Whole-cell catalysis is often a preferred choice if substrates and products can freely enter and exit the cells without being easily degraded by endogenous enzymes.

Enzyme Catalytic Reaction Condition Design and Optimization Platform

Based on mature experience, the optimization process for reaction conditions, including pH, temperature, metal ions, substrate concentration, enzyme dosage, reaction time, and preservation of whole cells/enzymes, can be completed within approximately 1-3 months.

Product Purification Platform

Similar to the enzyme purification and immobilization platform, BAS has developed a range of purification resins and immobilization resins suitable for industrial production. Common functional groups include NTA, IDA, epoxy, amino, and composite groups. Different resin materials can be selected based on protein properties and reaction conditions. Whole-cell catalysis is often a preferred choice if substrates and products can freely enter and exit the cells without being easily degraded by endogenous enzymes.

Conclusion

BAS Biotechnology (Shenzhen) Co., Ltd. specializes in the research, production, and sales of coenzyme products utilizing whole enzyme catalysis technology. With dedicated platforms for protein expression, enzyme modification, enzyme purification and immobilization, enzyme catalytic reaction condition design and optimization, and product purification, the company is committed to advancing the field of enzymology and providing high-quality solutions for various applications.

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